RESUMEN
Objective: This study was aimed at investigating the carrier rate of, and molecular variation in, α- and ß-globin gene mutations in Hunan Province. Methods: We recruited 25,946 individuals attending premarital screening from 42 districts and counties in all 14 cities of Hunan Province. Hematological screening was performed, and molecular parameters were assessed. Results: The overall carrier rate of thalassemia was 7.1%, including 4.83% for α-thalassemia, 2.15% for ß-thalassemia, and 0.12% for both α- and ß-thalassemia. The highest carrier rate of thalassemia was in Yongzhou (14.57%). The most abundant genotype of α-thalassemia and ß-thalassemia was -α 3.7/αα (50.23%) and ß IVS-II-654/ß N (28.23%), respectively. Four α-globin mutations [CD108 (ACC>AAC), CAP +29 (G>C), Hb Agrinio and Hb Cervantes] and six ß-globin mutations [CAP +8 (C>T), IVS-II-848 (C>T), -56 (G>C), beta nt-77 (G>C), codon 20/21 (-TGGA) and Hb Knossos] had not previously been identified in China. Furthermore, this study provides the first report of the carrier rates of abnormal hemoglobin variants and α-globin triplication in Hunan Province, which were 0.49% and 1.99%, respectively. Conclusion: Our study demonstrates the high complexity and diversity of thalassemia gene mutations in the Hunan population. The results should facilitate genetic counselling and the prevention of severe thalassemia in this region.
Asunto(s)
Hemoglobinopatías , Talasemia alfa , Talasemia beta , Humanos , Talasemia beta/epidemiología , Talasemia beta/genética , Talasemia alfa/epidemiología , Talasemia alfa/genética , Hemoglobinopatías/epidemiología , Hemoglobinopatías/genética , China/epidemiología , Secuenciación de Nucleótidos de Alto RendimientoRESUMEN
This project is to implement the computerization procedure of emergency nursing record. The nursing record must be complete and correct enough to present the uniqueness of different specialty. We encouraged nurses to participate in classroom lessons of informatics technology in nursing records. We then used the standardized version of the emergency nursing records. We also set up criteria for computerization of nursing records. As a result, the nursing records become more complete. It was improved by 8.5% as compared to manual records. The satisfaction of nurses was improved by 82.1%. It is much more time-saving and convenient to check the nursing records. In conclusion, the emergency nursing profession and quality surveillance did improve from the computerization. The patients will receive better nursing care quality.
